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Volume 11 Number 1 January 2025

Assessment of the Effects of TiwanTiwa Herbal Mixture Drink on Oxidative Stress Markers


Authors: Usiobeigbe O. S. ; Airhomwanbor K. O. ; Omolumen L. E., et.al.
Pages: 1-8
DOI: doi.org/10.32861/jbr.111.1.8
Abstract
Introduction: The use of herbal medicine and its effect on health has been established. Recently, there is much interest in therapeutic potential of herbal therapy use as antioxidant in reducing free radical-induced oxidative stress. Method: This study was aimed to determine the effect of herbal mixture on acetaminophen induced oxidative stress in male albino wistar rat. This is an experimental study comprising of total of forty-two (42) wistar rats divided into seven (7) groups of six (6) animals each. The seven groups were Group 1, control group fed with top feed and water only, Group 2 administered with 3000 mg acetaminophen and top feed, Group 3 with 3000 mg of acetaminophen and 140mg sylimarine, Group 4 with 3000 mg of acetaminophen and 50 mg tiwantiwa extract, Group 5 with 3000mg of acetaminophen and 100 mg tiwantiwa extract, Group 6 with 3000mg of acetaminophen and 200 mg tiwantiwa extract and Group 7 with 100 mg of tiwantiwa extract only. The treatment was given daily and lasted for 21 days, animals were sacrificed, and blood sample collected were assayed for oxidative stress; CAT, SOD, and MDA. Result obtained was analyzed with statistical package for social sciences (SPSS) version 20. Results: The result obtained depicts significant increase in CAT and SOD of group 5 (100mg of the extract) when compared to group 2 which is the toxic group induced with 3000mg Acetaminophen. There was also a significant decrease in MDA of group 5 (100mg of extract) when compared to group 2 (p<0.05). This implies that dose level (100 mg) of Tiwantiwa herbal mixture in group 5 exhibited the capacity to alleviate 3000 mg of acetaminophen toxicity at a minimal level. This is attributed to phytochemicals present in this extract such as phenolic compounds. Conclusion: 100 mg dose level of Tiwantiwa herbal mixture has a significant effect in alleviating 3000 mg of acetaminophen toxicity, acting as strong antioxidant as evidenced in the study with results shown in CAT, SOD and MDA bioassays. This is attributed to the presence compounds such as Phenols and Alkaloids which are potent phytochemical constituents in Tiwantiwa herbal mixture with antioxidant property.