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Volume 1 Number 4 October 2015

Comparison of Three Methods of DNA Extraction from Parachipteria willmanni (Acari: Oribatida) Collected in Turkey

Authors: Sedat Per ; Fahriye S?mer Ercan
Pages: 16-20
We compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocol that obtained from Parachipteria willmanni van der Hammen, 1952 mite specimens preserved in ethanol. The aim of the study is to find the most efficient protocol for obtaining DNA from Parachipteria willmanni. All methods are successful in isolating DNA from P. willmanni, but among these three methods, maximum amount of DNA is obtained with Chelex-method. DNAs obtained by these three methods were successfully applied to RAPD-PCR.