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Volume 6 Number 6 June 2020

In vitro Anti-Inflammation and Selective Cytotoxicity of Vero and HepG2 Cells by Phenolic Extract From Roots of Hermannia Geniculata Eckl and Zehl 


Authors: Adeniran Lateef Ariyo ; Ashafa Anofi Omotayo Tom
Pages: 61-66
DOI: doi.org/10.32861/ajls.66.61.66
Abstract
Anti-inflammatory and in vitro cytotoxic effect of phenols of Hermannia geniculata (PoHG) on Vero and HepG2 cells was carried out using Soybean lipoxygenase and MTT assays. PoHG extract exhibited a commendable inhibition of 5-lipoxygenase enzyme with IC50 value of (150 ± 0.03) µg/mL which is similar to the IC50: (110± 0.01) µg/mL of the standard (indomethacin). However, the extract was non-toxic to Vero cells with LC50 value >1.00 mg/mL but highly toxic to HepG2 cells (LC50: 0.05 mg/mL). A decrease viability of HepG2 cells was observed with increase in the concentration of the extract. There was less than 5% viable HepG2 cells at PoHG concentration of 750 µg/mL. The selectivity index of (20.00 and 33.33) was recorded for PoHG extract and doxorubicin respectively. The anti-inflammatory activities of PoHG suggested that the phenols extract may be useful in the management of inflammatory diseases like artheriosclerosis, diabetes mellitus, rheumatoid arthritis and asthma. It is also safe for use while its antiproliferative activities can be exploited in search for anticancer agents.

Protective Effect of Terminalia muelleri Extract on Brain of Streptozotocin-Induced Diabetes in Albino Rats


Authors: Sahar B. Ahmed ; Ghada Khiralla ; Shimaa Abdalla Harudy ; Hesham Elhariry
Pages: 53-60
DOI: doi.org/10.32861/ajls.66.53.60
Abstract
Diabetic neuropathy is one of the complications of diabetes. This study investigated the possibility of reducing neuropathy of STZ-induced diabetic rats by Terminalia muelleri extract (TE) and comparing the effect of the extract with the therapeutic effect of pioglitazone (PG) drug. The experimental animals were divided into non-diabetic (normal control), STZ-induced diabetic (diabetic control), TE-treated non-diabetic (200 mg/kg b.wt) (TE-group) TE-treated diabetic (200 mg/kg b.wt) (TE-STZ-group), and pioglitazone-treated diabetic (1.58 mg /kg b.wt) (PG-STZ-group). All treatments were administered orally by oral gavage once daily throughout the 4 weeks of the treatment period. In this study: malonaldehyde, nitric oxide, reduced glutathione and glutathione disulfide were examined as oxidative stress marker in the brain tissue of the experimental rats. The results indicated high oxidative stress in STZ-diabetic groups and reduced oxidative stress of groups treated with TE. The results of norepinephrine, dopamine, gammaamino- butyric acid, brain-derived neurotrophic factor, and Casps-3 also demonstrated the possibility of using TE to attenuate the effects of neuropathy in experimental rats comparable to PG use. This indicated that the TE is promising alternative to chemical treatment with PG drug. This indicated that TE is promising alternative to chemical treatment with PG drug.